Fig. 4.

Time course of FMDV replicon-derived GFP fluorescence. Data from mock-transfected BHK-21 cells are shown (×), together with cells transfected with transcript RNA derived from the pGFP-PAC replicon (◆), ‘leaderless’ replicon pLL-GFP-PAC (■) and polymerase deletion pGFP-PAC-Δ3D (▴) constructs. At the time points indicated images were captured and the GFP fluorescence quantified for each replicon construct: data shown as the green object count/mm² (Panel A) or the integrated GFP fluorescence intensity, ×10⁴ (Panel B). Data points/error bars shown are derived from three independent transfections, with four replicates for each transfection.