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. 2014 Oct 17;9(10):e109725. doi: 10.1371/journal.pone.0109725

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© 2014 Zhao et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Representative Western blots. α (90 kDa), β (95 kDa) and γ ENaC protein signals are found in blots probed with specific antibodies against α (Santa Cruz, sc-22239), β (Santa Cruz, sc-21013), and γ (Abcam, ab3468) subunits. B. Total expression levels of ENaC subunits. β actin is used as a loading control. C & D. Expression of ENaC proteins in ATI and ATII cells. The expression levels of ENaC proteins related to AQP5 (C) and pro-SPC (D) were adjusted to compensate for the alterations in AQP5 and pro-SPC (dotted bars). One-way ANOVA. *P<0.05 and ***P<0.001 versus control groups.