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. Author manuscript; available in PMC: 2015 Mar 11.
Published in final edited form as: N Engl J Med. 2014 Sep 3;371(11):1028–1038. doi: 10.1056/NEJMoa1315815

Figure 4. Detection of Full-Length Androgen Receptor mRNA and AR-V7 in Metastatic Prostate-Cancer Tissue.

Figure 4

In situ detection of full-length androgen receptor mRNA and AR-V7 in prostate-cancer tumor specimens was performed with the use of RNA in situ hybridization analysis. The tumor-tissue specimens shown are a radical-prostatectomy (RP) specimen that lacks AR-V7 expression from a patient (not enrolled in this study) who had not received hormonal treatment, an autopsy-derived specimen of a liver metastasis from a patient whose circulating tumor cells were shown to express AR-V7 (Autopsy), and core-needle biopsy specimens from a patient in whom AR-V7 was not detected (Biopsy 1) and a patient in whom AR-V7 was detected (Biopsy 2) in circulating tumor cells. All the tumor specimens show expression of full-length androgen receptor mRNA. The prostate-cancer cell lines that served as positive and negative controls for AR-V7 detection by means of RNA in situ hybridization are shown in Figure S6 in the Supplementary Appendix.