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A, Representative immunostaining for orexin/hypocretin neurons in hypothalamus. B, Quantification of orexin-IR and MCH-IR from separate series of hypothalamic sections processed using peroxidase immunohistochemistry. Mean and SEM numbers of orexin-IR and MCH-IR neurons in LH; C, Mean and SEM active and inactive nosepokes across extinction training; D, Mean and SEM number of active and inactive nosepokes on test for renewal (ABA) and extinction (ABB); *p<.05.
