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. 2014 Oct 17;9(10):e110544. doi: 10.1371/journal.pone.0110544

Figure 4. Generation of a Dictyostelium cln3 knockout mutant.

Figure 4

(A) Creation of a Dictyostelium cln3 knockout mutant by homologous recombination. The pLPBLP targeting vector and sites of recombination are shown. (B) Validation of cln3 knockout by PCR analysis. Primers are denoted by Roman numerals and arrows. The Dictyostelium gene denoted DDB_G0291155 lies downstream of cln3 and was amplified to confirm that the insertion of the bsr cassette did not affect this gene. (C) Validation of cln3 knockout by Southern blotting. DNA ladder (in bp) is shown to the left of the blot.