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. 2014 Oct 17;9(10):e110397. doi: 10.1371/journal.pone.0110397

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© 2014 Husain et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

The optic nerve (non-myelinated; 2 mm post globe) of Brown Norway rats was removed 7-days post glaucomatous injury. Contralateral optic nerve was used as the normal control. Cryosections were immunostained by anti-iNOS antibodies as indicated horizontally. Ocular treatments are indicated vertically. Green color indicates staining for iNOS and blue nuclei for DAPI. There was no positive staining when primary antibodies were omitted (data not shown). Data shown here are representations of at least four independent experiments. A total of 10 animals were used in this experiment. Comparable staining for iNOS was seen in at least 4 animals in each treatment group.