Figure 9. Changes in iNOS expression in glaucomatous retina at 7 and 42 days, post injury.
(A) Brown Norway rats were treated with SNC-121 (1 mg/kg, i.p.) 30 minutes after glaucomatous injury and continued for 7 days, once daily. Retina extracts were collected at the 7th day, post injury, and analyzed using anti-iNOS antibodies and appropriate secondary antibodies (HRP-conjugated; dilution 1∶3000). The signal was captured using enhanced chemiluminescent reagent and the Biorad Versadoc imaging system. Data are expressed as mean ± SEM. *P<0.05; n = 4. (B) The eyes of Brown Norway rats were removed 42-days post glaucomatous injury. Cryosections of retina were immunostained by anti-iNOS antibodies as indicated horizontally. Ocular treatments are indicated vertically. Data shown in this Figure are a representation of at least four independent experiments.