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. 2014 Oct 17;9(10):e110611. doi: 10.1371/journal.pone.0110611

Figure 6. Effect of codelivered cMLVs on P-gp expression in tumors.

Figure 6

(A, B) Mice bearing 4T1 tumor (A) and multidrug-resistant tumor JC (B) were injected intravenously through the tail vein with cMLV (5 mg/kg Dox), cMLV (5 mg/kg PTX), 5 mg/kg Dox + 5 mg/kg PTX, or cMLV (5 mg/kg Dox+5 mg/kg PTX). Three days after injection, tumors were excised, and stained by P-gp-specific antibody (green), followed by nuclear costaining with DAPI (blue). Scale bar represents 50 µm. (C, D) Quantification of P-gp-positive cells in 4T1 (C) and JC (D) tumors. To quantify P-gp-positive cells, 4 regions of interest (ROI) were randomly chosen per image at ×2 magnification. Within one region, area of P-gp-positive nuclei and area of nuclear staining were counted. The data are expressed as % total nuclear area that is P-gp-positive in the region. Data are represented as mean ± SD (n = 3).