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. Author manuscript; available in PMC: 2015 Apr 16.
Published in final edited form as: Nature. 2014 Aug 10;514(7522):372–375. doi: 10.1038/nature13590

Fig. 2. RIG-I associates with 5′-diphosphate-bearing viral RNAs.

Fig. 2

(a) Left, Experimental procedure. Right, IFN-β promoter reporter assay of RNA from RIG-I-precipitates. (b-c) (b, left) Experimental procedure using the following input RNA: reoT1L or reoT3D vRNA, reoT3D S or L segments, RNA isolated from reoT1L-infected cells, or total L-A RNA. (b, left) IFN-β promoter reporter assay or (b, right) ifnb1 expression from IFN-pre-treated MDA5−/− DCs (c) following transfection of RNA from RIG-I-precipitates treated +/−CIP. For PCR data, the mean (±s.d) of triplicate technical replicates is shown. All experiments were performed at least twice.