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. Author manuscript; available in PMC: 2015 Apr 16.
Published in final edited form as: Nature. 2014 Aug 10;514(7522):372–375. doi: 10.1038/nature13590

Fig. 3. De novo generated base-paired 5′-diphosphate RNA triggers RIG-I.

Fig. 3

(a) Experimental procedure used to generate 5′pp-IVT-RNA25nts. (b-c) IFN-β promoter reporter assay of IVT-RNA25nts+/−AS (b) and pp-IVT-RNA25nts+AS +/−CIP (c). (d-g) IFN-α levels (d,f,g) or ifit1 expression (e) from DCs (d) MEFs (e,g) or human PBMCs (f) transfected with indicated RNAs. Water, poly(dA:dT) or cyclic-di-GMP were included as controls (*not detected). For (f), the value obtained for 800ng/ml of [ppp-RNA24nts+AS] was set to 100%. Mean values (+s.d.) from four donors are shown (***P<0.0001). (h) AlphaScreen of RIG-I and synthetic RNA ligands (±s.d.). Units are proportional to RIG-I-ligand complex concentration. One experiment of two is shown.