Figure 3. Multivariate Analysis of Candidate Stability.

Animals were categorized as either TCDD-treated or control and separated into training and validation cohorts. All possible combinations of candidates were analyzed using the NormFinder algorithm. A lower value indicates less variance across all experimental conditions. (A) Combinations of candidates were organized according to the number of proteins included, in order to determine the optimal number of proteins used. (B) Stability results for each combination of candidates were compared between the training and validation sets to assess concordance. (C) Results for each combination of gene(s) were plotted for both the training (+) and validation (•) cohorts. Combinations are organized according to performance in the training set.