Table 2. Summary of analysis methods.

		NormFinder
	Student's t-test	Training	Validation	Normalization Method
ACTB	6/28	0.092	0.060	996.59
EEF1A1	11/28	0.112	0.050	278.40
GAPDH	5/31	0.072	0.077	316.07
HPRT	1/31	0.078	0.046	306.46
PGK1	6/29	0.144	0.081	259.58
PPIA	8/31	0.140	0.066	366.06
SDHA	10/26	0.071	0.056	286.62

Three analysis methods were used to evaluate the abundance consistency of each individual candidate protein; values in bold indicate the top ranked score for each method. 1) The difference between treated and untreated animals for each experimental condition was assessed by Student's t-tests; a p-value <0.05 was deemed significant. 2) The variation of each candidate was assessed using the NormFinder algorithm in two separate cohorts; a lower score indicates greater stability. 3) The comparative normalization method was used to evaluate the ability of each candidate to remove variation from a dataset; the average standard deviation for each pair-wise comparison is reported.