Table 3. Comparison between mRNA and protein abundances.
Spearman's correlation | ||
ρ | p-value | |
EEF1A1 | −0.02 | 0.79 |
GAPDH | 0.13 | 0.08 |
HPRT | 0.17 | 0.02 |
PGK1 | 0.11 | 0.14 |
PPIA | −0.15 | 0.04 |
SDHA | −0.06 | 0.46 |
Spearman's correlation was used to evaluate concordance between mRNA and protein abundances as determined by qPCR (mean Cq of technical replicates) and western blot (log2 of the protein intensity). Note that an increasing mRNA abundance results in a lower Cq; hence an inverse correlation indicates similarity between molecule abundance.