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. Author manuscript; available in PMC: 2014 Oct 17.
Published in final edited form as: Methods Mol Biol. 2009;556:191–203. doi: 10.1007/978-1-60327-192-9_14

Fig. 14.2. BrdU ELISA to check fractionation of H/L DNA.

Fig. 14.2

(A) Genomic DNA labeled during 0–2 h of S-phase. Gray line is the Anti-BrdU reactivity in an ELISA (measured as absorbance at 450 nm) and black line is the absorbance at 260 nm for total DNA. The H/L DNA forms a peak away from the bulk of the unreplicated DNA. (B) Second purification of H/L fractions. Complete overlap of BrdU peak with total DNA peak indicates purity of H/L DNA.