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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Dev Biol. 2014 Feb 24;389(2):160–172. doi: 10.1016/j.ydbio.2014.02.012

Fig. 3.

Fig. 3

DMRT1 over-expression via in ovo electroporation of embryonic female gonads. Immunostaining for the RCASBP viral antigen p27 (green) or DMRT1 (green) on E6.5 left female gonads electroporated with RCASBP-DMRT1 (magnification 10×). The left panel shows widespread infection of RCASBP-DMRT1 throughout the left gonad (Lg) and an absence in the control (un-electroporated) right gonad (Rg). The right panel shows that DMRT1 over expression correlates to the appearance of viral p27 antigen, as the Lg is DMRT1 positive whereas the Rg is negative for DMRT1 protein.