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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Am J Hematol. 2014 Jul 22;89(10):954–963. doi: 10.1002/ajh.23786

Figure 3. RNA interference inhibition of mTOR signaling reduces bone marrow erythroid colony-formation capacity.

Figure 3

A. Inhibition efficiency of short hairpin RNAs targeting S6 kinase was tested by transducing NIH3T3 cells with the indicated short hairpin RNAs and analyzing S6K1 expression by Western blot. B. Number of bone marrow BFU-E- and CFU-E-derived colonies formed by lineage negative cells transduced with short hairpin RNAs targeting S6 kinase or scrambled control are shown. Results shown are mean ± SEM of triplicates; *P < 0.05; **P < 0.01; ***P < 0.001; Student’s t test.