Table II.
Primer Sequences. “c” stands for custom i.e., self-designed, commercially synthesized primers and “ M” for M13 tailed primers. 19 bp M13 universal sequence (5′-CACGACGTTGTAAAACGAC) is written along with the marker specific forward primer sequences.
| Marker | Forward Primer | Reverse Primer |
|---|---|---|
| D21S1411c | TGTGTGTCCTTCCAGGCTTT | GTGGATAGAGAGATAGAACGGATAGAA |
| D16S3046c | AATAAACTGCGTGCGCTCAT | GTAATTTCATGGACCCCCTATTG |
| D17S1301c | ATGTGTATAACAAAATTCCTATGATGG | GGGTGACAGAGCAAGGTTCT |
| D15S968c | CAGTGCTTACCTGGGTCCAT | GCTCTATATTGGGGCCAAGC |
| D14S983c | ATTGGACTGGTTAGCCTCAGTG | GCATCAACTGGCTTCCAATC |
| D14S61c | CGGAGTCAATATGCTCTGGA | GGCGTATCAGAGAGGAAGGTT |
| D2S1245c | TGAAAGCTGGTGAATAAAGGG | GCCACTACAGCTTGCTTGC |
| USH1GU1 | AGCAAGCCCTTCTGCTCTCT | GCCAAAGTGGGAAGATCACT |
| D2S324c | GCACAAACTTTACATGCACACA | CCGATGTCTGGCATATCCTC |
| D9S158c | CATAAAGTAGGCAAAGCGGAAA | GTCCGTTGCCTCTGAAGATGT |
| C21SA2 | CGT TGTTGGCCTTCAGAT TT | GCT GTG AGG CCA GAG AGA AA |
| M D17S2207c | CACGACGTTGTAAAACGACCAGTGTTTACTGAGGCCTTTCTG | CGGTTGACGGAGTGAGACTG |
| M D15S988c | CACGACGTTGTAAAACGACGGATGAAAACATCTGGTTCAAA | TTCGGTTTAAGAATGATTGTTCC |
| D21S167c | ACCAGCTTCAAACGTGCAG | GCATCAGTGCTCTGTTTGGTT |
| D10S1775c | CCTCCCCTAGTTAATGGATGT | GATTTTCACAGGTATTGCTGAGT |
| M D15S967c | CACGACGTTGTAAAACGACTCAAGGTGAGACACCACAGG | CATACAGGACCCCAGGAAGA |
| D10S1643c | CCTTAATCACATAAGCCAATTTCTGA | CCACTTCTCAGTGCCAAAATCT |
| M D4S2369c | CACGACGTTGTAAAACGACTGGCATGAGCAACCTTACAG | CTCCAGACTGGGCGATAGAG |
| USH3Acc | CGTTACTTAATGCCATATCCCTA | TCAGGCCCAGGGTACTTT |
| D15S1025c | CACGACGTTGTAAAACGACATGCCCTTGCTGATGTTTTC | GCAAGGTGCAGAACAATATGC |
| M D12S2074cFN | CACGACGTTGTAAAACGACAGGTGGTCTGTAGGCTACGTG | CCAAACCCCAAACCATACAT |
| D11S4138c | CCAGGGTTAGTGTGGGAGAG | GGTCAGACTGAGGTGAGAAGG |
| D10S606c | AAGACATCGCAACATGGACA | TTGTTTGAACCTGGGAGACG |
| D11S911c | CTCTTCTCATGCTTGACCATTT | CCTTCTGAACAATTGCCACAT |
| M D12S1708c | CACGACGTTGTAAAACGACGGAAGCATGCCTTTTGAAAT | ACAGACATGCACCACCACAC |
| M C6SU1 | CACGACGTTGTAAAACGACGTGCCAGGAACCAAGGACTA | GAGGCTGAGGCACAAGAATC |
| M C6SD1 | CACGACGTTGTAAAACGACCCAGAGCTGAGAAAGAACAAA | GCCTGAGTGACAGAGTGAGACT |
| D9S1837c | CAGAAATGGCAGCTGGTGT | GTTCATGATGGTGGTCTCTGG |
| D9S1876c | TAGTGGTTACCATTTACCCAAGA | AAGATGTACCCAGAGAAGTCTCG |
| D11S4186c | CCAGATTATTCTCCCAATCTATCG | GCACGTGCTGTGCAGTGTA |
| M D4S3357c | CACGACGTTGTAAAACGACTGCTTCAAAGAGGAATTGATGA | GGCTGATTAGTATGCCATTGGA |
| D10S1759c | ACCTACTCCCTGCACAACTGG | GAATGGGCACGCTCACAC |
| D11S1314c | AAGGCAGGAAATGTGACCTC | GGTTGCACTTAGACATGCACA |
| D5S618c | TGATTGAAGGATGCCAAATTAC | GGTGACCAAGGCTTCACAG |
| GATA123E07c | TCAGTGATGGCATAGCTAGATAGAA | TTTTGAGGTCTATCCATGTTGG |
| D10S89c | CTGAAGGCTTCTGGAGCA | GGCATAAGTTAAGAACACTAGTGACA |
| D7S2504c | AGGCCACCACAATGTCAGTT | GCAATGAGAGATGCTTGGAATA |
| D10S197c | CCTCAAGTGGCATTGTGAAA | GTTGCAGTGAGCTGAGATCG |
| M D1S441c | CACGACGTTGTAAAACGACTAATTGCACCACTGCACTCC | TTAAAGATTAGCCGAGCATGG |
| D1S2629c | TCCAACAGCAGATAAGTGGATG | CCAATTCCATTCATGTTACTGTCA |
| D16S403c | GAGCCTCAGGCCTTTATGA | GTTTTCTCCCTGGGACATTT |
| D11S902c | CCCGGCTGTGAATATACTTAATG | CCAACAGCAATGGGAAGTT |
| D5S815c | CTTGTGTAGCAAATTACAGCTTACA | TCTGCCATGATTGTTAAGTTTCC |
| D7S2448c | CCTGGAATCACAGCCTCATAA | GTTCCCCAGGCTCCAACT |
| D10S546c | GACTCCAGCCTTTCACACCT | GGCAAAGTTGATGCTGCTTA |
| D11S4139c | TCTTTGATTTGCTGGAAGATGC | GTCACACGTGCACACACTCTTAC |
| D9S1824c | CTTCCTCTCTGGTCTCCCTGT | GCTCCCCTGGATTTCCTAA |
| D1S229c | TGGTATGGACTCCAGTCAGG | GGCACACAATTGTCCCTACA |
| D2S174c | TTAGAGCACACATGGTCACTCC | GCTCCAGAAGTGGCTACAGG |
| D6S1031c | CAGCACTCACATGCTTATCCTTT | GAATCCGTCTCACAGGCTCA |
| D6S456c | CAGCACTCACATGCTTATCCTTT | GAATCCGTCTCACAGGCTCA |
| M D12S1686c | CACGACGTTGTAAAACGACATTGGAGGACCCACAGTCAG | ATGCTTCTGCTTGACCTGCT |
| M C3SB42A | CACGACGTTGTAAAACGACTTGAGGTCTGTGAGTGGCTTC | GAGGTTGAAGTGAGCGGAGA |
| D1S2870c | ATAGGTGTGAGCCACTGCAC | GCCTGGGCAGATACTTAGGA |
| D2S2223c | CCTCCCTTGATTGCTTTGAA | CCTGAAATGTTTGGCGATTT |
| GATA129G03c | TCAGATTATGTTCTCATACAGCCTTT | GAAACTGGGCAGAGGCATAG |
| M D1S417c | CACGACGTTGTAAAACGACCTGAGGTCTGATCCCTCTGG | AGGCTGTACCATTTAGGTTCG |
| M D1S2652c | CACGACGTTGTAAAACGACCCAATGAGACCAAGCAGGAT | GAAAATGGCACATGTTGTGG |
| M C19SA2 | CACGACGTTGTAAAACGACTCTCATTGGGGTCCTTCTTG | GGGCAACAAGAGCGAAACT |
| M C19SA3 | CACGACGTTGTAAAACGACTCCATCCTTGCCTTAACACC | GAGGCAGAGGTTCCTGTGAG |
| D6S1618c | CCATGGCATCTGTCCTTCC | GCTCACACTGGCTTCTCCTT |
| D10S1265c | AGAATGGCCAGTACAAACAATC | GTAATTTCCATTGCTGACAGG |
| M D12S75c | CACGACGTTGTAAAACGACGAAGCAGAAGCTGCCAGAAT | CCTACCTTGATCCGACCTGA |
| M D3S4019c | CACGACGTTGTAAAACGACGGTGGTGGGCGCCTGTAGT | ACCCAGAAAGGGGTCCCAAG |
| D22S272c | AATGCCTTCAGCATGGACCT | GGCATACAGGAGCCTAGACAGG |
| GATA64B01c | TGCCTAATTTTCCTGTATTGTTCC | AATTAGCTGGGTGTGGTGGT |
| M D1S475c | CACGACGTTGTAAAACGACATGGTTACGTGAGCCCAGTC | CACTCCCCTTCAGACTGCTG |
| D6S1285c | AAAGCACTCTGAACACAGATAATGTAA | TTTGGCCTAGCTCTTTCACC |
| M D7S660c | CACGACGTTGTAAAACGACTTCCAATAGGCCAACACTGG | GAAACATAATATCCAACATGGTGAC |
| M D7S802c | CACGACGTTGTAAAACGACATGCAGAGAAACCTTGTGAGA | ACATAGGCCCCCAATCTCTT |
| D22S1045c | TGCAGGCCTCTTAGGATCTG | GTGTGACCTCAGGCAAGTCC |