Table IV.
Pools of STR Markers. The marker labels are placed as prefixes and “M” is acronym for universal M13 sequence tailed primers. The self-designed primers and LMG, NIH provided primers can be distinguished in the table by “c” (custom) suffixes for the self-designed ones. The “+” sign is placed between two markers multiplexed together.
| Pool 1 |
| FAM D1S248+D9S1838 |
| FAM SN18–27 |
| FAM D1S3723R2* |
| VIC D21S1411c+D7S2459 |
| VIC D16S3046c+D21S1225 |
| NED D17S1301c |
| NED D15S968c |
| NED B16Set2** |
| NED D7S496* |
| PET D14S983c+ D14S61c |
| PET D2S1245c |
| Pool 2 |
| FAM D1S495+USH1GU1 |
| NED D17S2206+M D2S324c |
| VIC D9S158c+HEX C21SA2 |
| M HEX D17S2207c |
| PET D21S167c+M D15S988c |
| FAM D11S4107+ D7S2420 |
| NED D21S1252 |
| Pool 3 |
| FAM SN1–141.48B+D1S2730 |
| FAM D10S1775c+M D1S967c |
| NED D10S1643c+B16SET4 |
| M HEX D4S2369c |
| VIC USH3ACc+ M D15S1025c |
| M PET D12S2074cfn |
| Pool 4 |
| PET D11S1438c+D10S606c |
| PET D11S911c |
| FAM D5S637+M D12S1708c |
| FAM D9S1837c +M C6SU1 |
| NED D9S1876c+D11S4186c |
| NED D11S925 |
| VIC D10S1759c+M HEX |
| D4S3357c |
| VIC D11S1314c |
| M HEX C6SD1*** |
| Pool 5 |
| FAM D5S1463+SN18–36 |
| NED OTOA55U+D5S618c |
| VIC GATA123E07c |
| VIC D10S89c+D7S2504c |
| PET D10S197c*** |
| FAM D5S629 |
| M FAM D1S441c**** |
| Pool 6 |
| NED D1S2629c+D16S403c |
| FAM D11S902c+D5S815c |
| NED D7S2448c+D10S546c |
| FAM D11S4139c |
| VIC D9S1824c+D1S2827 |
| PET D1S229c+D3S1594 |
| Pool 7 |
| VIC D6S1560+D2S174c |
| VIC D6S1031c+D6S456c |
| FAM D3S1767+M D12S1686c |
| M FAM C3SB42A***** |
| FAM D1S2870c |
| NED D2S2223c+GATA129G03c |
| M PET D1S417c |
| M PET D1S2652c |
| M NED C19SA2+C19SA3 |
| Pool 8 |
| FAM D6S1686c+D10S1265c |
| M FAM D12S75c+ D3S4019c |
| VIC D1S3774+D22S272c |
| VIC GATA64B01c****** |
| NED D3S3582+M D1S475c |
| PET D6S1285c+M D7S660c |
| PET D22S1045c +M D7S802c |
An asterisk sign marks exceptional conditions required for amplification.
*
65–53 touchdown, (NH4)2SO4 Buffer, MyCycler-(Bio-Rad),
**
70–55 touchdown, 3.5Mm MgCl2, MyCycler-(Bio-Rad,),
***
68–55touchdown 1.5mM MgCl2, Mastercycler (Eppendorf),
****
65–57touchdown, MyCycler-(Bio-Rad,),
*****
65–53touchdown, 1.5mM MgCl2,
******
(NH4)2SO4 Buffer, Mastercycler (Eppendorf).