. Author manuscript; available in PMC: 2014 Oct 17.

Published in final edited form as: Anal Chem. 2011 Jul 13;83(15):5957–5964. doi: 10.1021/ac201321m

Table 2.

Comparison of the Intensity and Cluster Ratios Derived from the Strand Exchange Reaction of G-S_A/S_B^s-TG₃ with S_B^a

[Cluster-G-S_A/S_B^s-TG₃] (nM)	Intensity Ratio	Number Ratio
1.8 ± 0.1	1.7 ± 0.4	1.8 ± 0.2
5.0 ± 0.3	2.5 ± 0.4	2.0± 0.1
9.8 ± 0.4	2.4 ± 0.3	2.7 ± 0.1
11.5 ± 0.6	2.0 ± 0.2	2.5 ± 0.1
27.0 ± 0.3	2.7 ± 0.2	3.2 ± 0.1

Based on FCS studies, the first column provides the concentration of near-infrared emissive clusters associated with G-S_A/S_B^s-TG₃.

The last two columns provide the ratio of the integrated emission intensity (second column) and the numbers of emitting species (third column) before vs. after the reaction S_B with the cluster conjugate with G-S_A/S_B^s-TG₃.