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. 2014 Oct 16;14:552. doi: 10.1186/s12879-014-0552-x

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© Venturini et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Determination of IL-1β, IL-6, TNF-α, MIP-1α, IL-10, TGF-β1 and FGFb levels in the cell-free supernatant obtained from monocytes from healthy subjects (CG, n =6), PCM-p before treatment (NTG, n =7) and PCM-p with apparent cure (ACG, n =8). The cells were cultured in the absence (spontaneous production – unstimulated) or presence of lipopolysaccharide - LPS and P. brasiliensis antigen - AgPb (stimulated production). Values are expressed as the mean ± standard deviation and comparisons were performed by ANOVA with Tukey's post-test. Different letters indicate significant differences among the groups (p ≤0.05).