Table 2.

Summary of the primers and PCR-RFLP conditions used for the analysis of the APOB polymorphisms

SNP (RS#)	Primer sequence	Amplification conditions	PCR prod (bp)	RE digests	Ref
SP Ins/Del (rs11279109)	5’-CAGCTGGCGATGGACCCGCCGA-3’	95°C 5 mins	93/84	N/A	Xu et al. [41]
		95°C 0.50 sec, 65°C 1 min,
	5’-ACCGGCCCTGGCGCCCGCCAGCA-3’	65°C 0.50 sec (35 cycles)
		72°C 7 mins, hold at 4°C
Codon 2488 Xba1 (rs693)	5’-GATGAAACCAATGACAAAATCC-3’	94°C 5 mins	450	240	Pan et al. [31]
	5’-AACAGTGAACCCTTGCTCTACC-3’	94°C 0.30 sec, 58°C 30 sec,
		70°C 0.30 sec(40 cycles)		210
		70°C 10 mins, hold at 4°C
Codon 3611 Msp1 (rs1801701)	5’-AGAACATACAAGCAAAGCCA-3’	94°C 5 mins	273	169	Pan et al. [31]
	5’-GAGGAACCTTAGGTGTCCTTC-3’	94°C 0.30 sec, 56°C 30 sec,
		70°C 0.30 sec(40 cycles)		104
		70°C 10 mins, hold at 4°C
Codon 4154 EcoR1 (rs1042031)	5’-TAGGCAAATTGATGATATCGA-3’	94°C 5 mins	330	181	Pan et al. [31]
	5’-ACCTGGGACAGTACCGTCCCTA-3’	94°C 0.30 sec, 54°C 30
		sec,70°C 0.30 sec(40 cycles)
				149
		70°C 10 mins, hold at 4°C
3’VNTR	5’-6- FAM- ATGGAAACGGAGAAATTATG-3’	95°C 5 mins	500-900	N/A	Alonso et al. [30]
		94°C 1 min, 58°C 1 min,
	5’-CCTTCTCACTTGGCAAATAC-3’	72°C 4 mins (29 cycles)
		72°C 7 mins, hold at 4°C