Table 5.
Distribution of genotype and allele frequencies of the APOB polymorphisms with the major variables analyzed among the Kuwaiti population (n = 795)
| TC | TG | HDL-C | LDL-C | FH-HC | FH-HTG | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Normal (≤5.17) | High (>5.17) | Normal (≤1.70) | High (>1.70) | Normal (>1.1 men & >1.29 women) | Abnormal (≤1.1 men & ≤1.29 women) | Normal (≤3.2) | High (>3.2) | Absent | Present | Absent | Present | |
| n | 475 | 191 | 571 | 95 | 211 | 418 | 389 | 238 | 409 | 197 | 594 | 12 |
| APOB signal peptide (rs11279109) | ||||||||||||
| II n (%) | 277 (58.3) | 118 (61.8) | 338 (57.4) | 67 (70.5) | 118 (55.9) | 258 (61.7) | 233 (59.9) | 141 (59.2) | 248 (60.64) | 121 (61.42) | 364 (61.28) | 5 (41.67) |
| ID n (%) | 172 (36.2) | 59 (30.9) | 210 (36.8) | 21 (22.1) | 81 (38.4) | 135 (32.3) | 134 (34.5) | 82 (34.5) | 138 (33.7) | 63 (32.0) | 198 (33.3) | 3 (25.0) |
| DD n (%) | 26 (5.5) | 14 (7.3) | 33 (5.8) | 7 (7.4) | 12 (5.7) | 25 (6.0) | 22 (5.7) | 15 (6.3) | 23 (5.6) | 13 (6.6) | 32 (5.4) | 4 (33.3) |
| p | 0.338 | 0.021 | 0.313 | 0.944 | 0.839 | 0.004 | ||||||
| D (f) | 0.236 | 0.228 | 0.242 | 0.184 | 0.249 | 0.221 | 0.229 | 0.235 | 0.225 | 0.226 | 0.221 | 0.458 |
| I (f) | 0.764 | 0.772 | 0.758 | 0.816 | 0.751 | 0.779 | 0.771 | 0.765 | 0.775 | 0.774 | 0.779 | 0.542 |
| p | 0.754 | 0.083 | 0.274 | 0.791 | 0.970 | 0.006 | ||||||
| APOB Codon 2488C (rs693) | ||||||||||||
| X − X − n (%) | 239 (50.3) | 104 (54.5) | 292 (51.1) | 51 (53.7) | 111 (52.6) | 217 (52.6) | 206 (53.0) | 122 (51.3) | 226 (55.3) | 106 (53.8) | 326 (54.9) | 6 (50.0) |
| X + X- n (%) | 199 (41.9) | 73 (38.2) | 234 (41.0) | 38 (40.0) | 84 (39.8) | 168 (40.2) | 152 (39.1) | 98 (41.2) | 153 (37.4) | 79 (40.1) | 229 (38.6) | 3 (25.0) |
| X + X + n (%) | 37 (7.8) | 14 (7.3) | 45 (7.9) | 6 (6.3) | 16 (7.6) | 33 (7.9) | 31 (8.0) | 18 (7.6) | 30 (7.3) | 12 (6.1) | 39 (6.6) | 3 (25.0) |
| p | 0.625 | 0.826 | 0.982 | 0.871 | 0.741 | 0.041 | ||||||
| X − (f) | 0.713 | 0.736 | 0.716 | 0.737 | 0.725 | 0.720 | 0.725 | 0.718 | 0.740 | 0.739 | 0.742 | 0.625 |
| X + (f) | 0.287 | 0.264 | 0.284 | 0.263 | 0.275 | 0.280 | 0.275 | 0.282 | 0.260 | 0.261 | 0.258 | 0.375 |
| p | 0.399 | 0.559 | 0.851 | 0.805 | 0.969 | 0.198 | ||||||
| APOB Codon 3611–MspI G (rs1801701) | ||||||||||||
| M + M + n (%) | 407(85.7) | 172(90.1) | 494(86.5) | 85(89.5) | 180(85.3) | 368(88.0) | 337(86.6) | 209(87.8) | 362(88.5) | 162(82.2) | 516(86.9) | 8(66.7) |
| M + M − n (%) | 65(13.7) | 18(9.4) | 75(13.1) | 8(8.4) | 30(14.2) | 47(11.2) | 49(12.6) | 28(11.8) | 45(11.0) | 35(17.8) | 76(12.8) | 4(33.3) |
| M − M − n (%) | 3(0.6) | 1(0.5) | 2(0.4) | 2(2.1) | 1(0.5) | 3(0.7) | 3(0.8) | 1(0.4) | 2(0.5) | 0(0.0) | 2(0.3) | 0(0.0) |
| p | 0.315 | 0.058 | 0.532 | 0.822 | 0.046 | 0.113 | ||||||
| M − (f) | 0.075 | 0.052 | 0.069 | 0.063 | 0.076 | 0.063 | 0.071 | 0.063 | 0.060 | 0.089 | 0.067 | 0.167 |
| M + (f) | 0.925 | 0.948 | 0.931 | 0.937 | 0.924 | 0.937 | 0.929 | 0.937 | 0.940 | 0.911 | 0.933 | 0.833 |
| p | 0.143 | 0.761 | 0.407 | 0.600 | 0.063 | 0.079 | ||||||
| APOB Codon 4154G (rs1042031) | ||||||||||||
| E + E + n (%) | 383(80.6) | 154(80.6) | 461(80.7) | 76(80.0) | 174(82.5) | 334(79.9) | 319(82.0) | 187(78.6) | 321(78.5) | 158(80.2) | 468(78.8) | 11(91.7) |
| E + E − n (%) | 83(17.5) | 35(18.3) | 102(17.9) | 16(16.8) | 34(16.1) | 77(18.4) | 63(16.2) | 48(20.2) | 82(20.0) | 33(16.8) | 114(19.2) | 1(8.3) |
| E − E − n (%) | 8(1.9) | 2(1.1) | 8(1.4) | 3(3.2) | 3(1.4) | 7(1.7) | 7(1.8) | 3(1.3) | 6(1.5) | 6(3.0) | 12(2.0) | 0(0.0) |
| p | 0.723 | 0.455 | 0.743 | 0.406 | 0.289 | 0.543 | ||||||
| E − (f) | 0.106 | 0.102 | 0.103 | 0.116 | 0.095 | 0.109 | 0.099 | 0.113 | 0.115 | 0.114 | 0.116 | 0.042 |
| E + (f) | 0.894 | 0.898 | 0.897 | 0.884 | 0.905 | 0.891 | 0.901 | 0.887 | 0.885 | 0.886 | 0.884 | 0.958 |
| p | 0.820 | 0.604 | 0.441 | 0.416 | 0.971 | 0.257 | ||||||
| APOB 3 VNTR | ||||||||||||
| MM n (%) | 391(82.3) | 157(82.2) | 466(81.6) | 82(86.3) | 174(82.5) | 345(82.5) | 321(82.5) | 197(82.8) | 339(82.9) | 168(85.3) | 496(83.5) | 11(91.7) |
| ML n (%) | 68(14.3) | 30(15.7) | 88(15.4) | 10(10.5) | 30(14.2) | 62(14.8) | 56(14.4) | 35(14.7) | 56(13.7) | 22(11.2) | 77(13.0) | 1(8.3) |
| LL n (%) | 16(3.4) | 4(2.1) | 17(3.0) | 3(3.2) | 7(3.3) | 11(2.6) | 12(3.1) | 6(2.5) | 14(3.4) | 7(3.6 | 21(3.5) | 0(0.0) |
| p | 0.633 | 0.461 | 0.875 | 0.916 | 0.685 | 0.700 | ||||||
| L (f) | 0.105 | 0.099 | 0.107 | 0.084 | 0.104 | 0.100 | 0.103 | 0.099 | 0.103 | 0.091 | 0.100 | 0.042 |
| M (f) | 0.895 | 0.901 | 0.893 | 0.916 | 0.896 | 0.900 | 0.897 | 0.901 | 0.897 | 0.909 | 0.900 | 0.958 |
| p | 0.754 | 0.343 | 0.834 | 0.816 | 0.537 | 0.503 | ||||||
TC total cholesterol (expressed in mmol/L); TG triglycerides (expressed in mmol/L); LDL-C low density lipoprotein-cholesterol; HDL-C high density lipoprotein-cholesterol; FH-HTG positive family history of hypertriglyceridemia, FH-HC positive family history of hypercholesterolemia.
n = number of samples,% = frequency for each genotype in percent and ratio (f) for the allele.
The p-values were determined by chi-square test at a significance level of p = 0.05.