Table 2.
Molecular identification of isolated strains, their nearest neighbors based on 16S rRNA and phaC/phaC1 genes and their isolation location
| Isolate | Putative PHA | Closest relatives based on 16S rDNA sequence (Accession no/identity %)* | Closest cultured based on phaC/phaC1 gene sequence (Accession no/identity %)* | Isolation source |
|---|---|---|---|---|
| Pseudomonas sp. P1 | Mcl | Pseudomonas extremaustralis SY11 (KC790323/99) | Pseudomonas fluorescens Pf0-1 (CP000094/91) | Mat (pond 10) |
| Pseudomonas sp. P4 | Mcl | Pseudomonas frederiksbergensis (JF343187/100) | Pseudomonas fluorescens Pf0-1 (CP000094/90) | Mat (pond 10) |
| Pseudomonas sp. P19 | Mcl | Pseudomonas putida C14 (JN228297/99) | Pseudomonas fluorescens BM07 (FJ472656/91) | Water (pond 11) |
| Pseudomonas sp. P27 | Mcl | Pseudomonas antarctica (HE586386/99) | Pseudomonas fluorescens SBW25 (AM181176/95) | Water (pond 8) |
| Pseudomonas sp. P32 | Mcl | Pseudomonas putida (HE586397/99) | Pseudomonas sp. KBOS04 (AY790328/92) | Water (pond 8) |
| Pseudomonas sp. P45 | Mcl | Pseudomonas fragi (AB685634/100) | Pseudomonas stutzeri 1317 (AY278219/84) | Stream 6 |
| Janthinobacterium sp. P10 | Mcl | Janthinobacterium lividum (HQ824865/100) | Pseudomonas extremaustralis (FN435843/90) | Water (pond 10) |
| Janthinobacterium sp. P24 | Mcl | Janthinobacterium lividum (HQ824864/100) | Pseudomonas fluorescens BM07 (FJ472656/92) | Mat (pond 10) |
| Antarctic Betaproteobacterium P21 | Scl | Burkholderia sp. Era35(JQ977167/99) | Zooglea ramigera (U66242/83) | Water (pond 7) |
| Antarctic Betaproteobacterium P25 | Scl | Rhodoferax ferrireducens (HG003356/99) | Comamonas testosteroni CNB-2 (CP001220/81) | Water (pond 10) |
| Janthinobacterium sp. P38 | Scl | Janthinobacterium sp. SMN 33.6 (JX624164/99) | Zooglea ramigera (U66242/83) | Water (pond 10) |
| Janthinobacterium sp. P39 | Scl | Janthinobacterium sp. TMT4-26-6 (JX949991/99) | Zooglea ramigera (U66242/83) | Water (pond 7) |
| Pseudomonas sp. P5 | - | Pseudomonas fragii (AB685683/99) | - | Water (pond 8) |
| Iodobacter sp P11 | - | Iodobacter fluviatilis (KC213858/100) | - | Water (pond 7) |
| Flavobacterium sp. P14 | - | Flavobacterium algicola (AB455265/99) | - | Water (pond 10) |
| Yersinia sp. P16 | - | Yersinia intermedia (NR_027545/99) | - | Water (pond 7) |
| Shewanella sp. P28 | - | Shewanella putrefaciens (KC607503/99) | - | Water (pond 8) |
| Janthinobacterium sp. P30 | - | Janthinobacterium lividum (HQ824941/99) | - | Water (pond 7) |
| Antarctic Alphaproteobacterium P35 | - | Sphingomonadaceae bacterium N (DQ497241/99) | - | Water (pond 7) |
| Antarctic Betaproteobacterium P36 | - | Rhodoferax ferrireducens (NR_074760/99) | - | Water (pond 8) |
* Only cultured strains were considered during BLAST searching