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. 2014 Oct 16;15(1):120. doi: 10.1186/s12931-014-0120-3

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© Li et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Y-FISH and SPC double-immunofluorescence staining in lung tissues. (A) Control group (group I). Y-FISH staining (red) was negative. Nuclei were counterstained with DAPI (blue). (B) Emphysema group (group II). Y-FISH staining was again negative. (C) Emphysema + rAFMSC transplantation for 20 days (group III). Y-FISH staining was weakly positive and a few Y-FISH-positive cells were also positive for SPC staining (green). (D) Emphysema + rAFMSC transplantation for 40 days (group IV). The number of Y-FISH-positive cells was increased substantially, and the number of Y-FISH and SPC double-positive cells was markedly higher than that in group III. (E) Positive-control group (male rats). Y-FISH staining was strongly positive. Cells were counterstained with DAPI. (F) The percentage of Y-FISH-positive cells in each group. (G) The percentage of Y-FISH and SPC double-positive cells in each group. Values are presented as average percentages of positive cells (means ± SEM, n = 10); *P < 0.01 versus group I, ^# P < 0.05 versus group III. Scale bars, 40 μm.