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. 2014 Oct 16;15(1):120. doi: 10.1186/s12931-014-0120-3

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© Li et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Phenotypes and Oct-4 mRNA expression of rAFMSCs. (A) The rAFMSCs were phenotypically characterized by means of flow cytometry. The relevant isotype controls were used as the negative control. The surface antigens CD29, CD44, CD73, CD90, CD105, and CD166 were expressed in P3 rAFMSCs, but CD14, CD19, CD34, and CD45 were not. (B) Oct-4 mRNA expression in rAFMSCs was analyzed by performing RT-PCR. Lane 1, rAFMSCs; Lane 2, negative control (rat lung fibroblasts).