Table 1.
Examples of significantly enriched GO annotation for the list of morphine-responsive genes
| Enriched GO annotation | De‐regulation | Up‐regulation | Down‐regulation | |||||
|---|---|---|---|---|---|---|---|---|
| No. genes | P value | No. genes | P value | Genes | No. genes | P value | Genes | |
| Oxidation‐reduction process (GO:0055114) | 52 | 3.4E + 10 | 18 | 0.013 | acox1, cyp11a1, cyp26b1, cyp27a1.4, d2hgdh, gapdhs, | 34 | 3.7E + 10 | adh8b, aldh18a1, cp, cyp2aa4, dao.1, dhfr, dhrs13b, mdh1b, phyhd1, ptgr1, sepx1a |
| Proteolysis (GO:0006508) | 37 | 0.049 | 11 | ube2d2 | 27 | 0.036 | f7, rad23b, spcs2, uchl5, vsg1 | |
| Macromolecule catabolic process (GO:0009057) | 23 | 0.003 | 4 | ube2d2 | 19 | 4.3E + 12 | ctbs. LOC553461, rad23b, uvsg1 | |
| Monosaccharide metabolic process (GO:0005996) | 18 | 6.8E + 08 | 11 | 3.8E + 09 | aldocb,galk2, gapdhs, gpia | 7 | 0.050 | mdh1b, pklr |
| DNA metabolic process (GO:0006259) | 17 | 0.080 | 5 | mlf1, mphosph8 | 12 | 0.054 | orc4, rad23b | |
| Protein catabolic process (GO:0030163) | 16 | 0.002 | 16 | 0.002 | LOC553461, rad23b, uchl5, vsg1 | |||
| Macromolecular complex assembly (GO:0065003) | 16 | 0.003 | 7 | 0.044 | h1fx | 9 | 0.065 | fgg, tubb5 |
| Generation of precursor metabolites and energy (GO:0006091) | 15 | 0.012 | 9 | 0.006 | aldocb, gapdhs, gpia | 6 | mdh1b, pklr | |
| Homeostatic process (GO:0042592) | 15 | 0.025 | 5 | fth1 | 10 | 0.060 | ||
| Posttranslational modification. protein turnover. chaperones | 13 | 0.005 | 1 | 12 | 1.9E + 12 | |||
| Protein complex assembly (GO:0006461) | 12 | 0.005 | 4 | 8 | 0.024 | fgg, tubb5 | ||
| Lipid metabolism | 9 | 0.005 | 4 | 0.087 | acsl1, acsl5 | 5 | 0.072 | |
| Response to wounding (GO:0009611) | 9 | 0.010 | 3 | cxcl12a | 6 | 0.042 | f7, fgg | |
| Proteasome complex (GO:0000502) | 8 | 5.1E + 11 | 8 | 5.1E + 11 | ||||
| Blood coagulation (GO:0007596) | 7 | 7.1E + 11 | 2 | tfpia | 5 | 0.005 | f7,fgg | |
| Positive regulation of apoptosis (GO:0043065) | 7 | 0.010 | 3 | bnip4 | 4 | |||
| Striated muscle cell development (GO:0055002) | 6 | 0.060 | 3 | cxcl12a | 3 | tnnt2a | ||
| Intermediate filament cytoskeleton (GO:0045111) | 5 | 0.043 | 1 | cyp27a1.4 | 4 | 0.043 | krt23 | |
| Notch signaling pathway (GO:0007219) | 5 | 0.072 | 2 | dlc | 3 | vsg1 | ||
| ribosomic RNA processing (GO:0006364) | 5 | 0.086 | 5 | 0.021 | ||||
| Secondary metabolites biosynthesis. transport. and catabolism | 5 | 0.044 | 5 | 0.044 | cyp11a1. cyp26b1. cyp51 | |||
| protein processing (GO:0016485) | 4 | 0.036 | 1 | 3 | spcs2 | |||
| serine family amino acid metabolic process (GO:0009069) | 4 | 0.065 | 2 | gcshb | 2 | dhfr | ||
The complete results of the Gene Ontology (GO) analysis are presented in Additional file 3.