Table 4.
Comparison between microarray and RT-qPCR data for selected genes
| Transcripts cluster Id | Gene names | Microarray data R.fold | RT‐qPCR data | |
|---|---|---|---|---|
| Fold change | P‐value | |||
| Up‐regulation | ||||
| Dr.24320.1.S1_at Dr.21771.1.S1_at | bnip4 | 2.36/1.23 | 1.90 ± 0.41* | 7.42 E‐05 |
| Dr.9852.1.A1_at | camk1γb | 2.16 | 1.90 ± 0.57* | 1.41 E‐05 |
| Dr.7717.2.A1_at Dr.7717.1.A1_at | grb2 | 2.02/1.58 | 1.27 ± 0.15* | 8.98 E‐04 |
| Dr.3576.1.A1_at | acox1 | 1.74 | 2.03 ± 0.43* | 2.04 E‐05 |
| Dr.4477.1.A1_at | magi1b | 1.30 | 1.55 ± 0.12* | 5.43 E‐05 |
| Dr.3645.1.S1_at | plg | 1.26 | −1.06 ± 0.21 | 0.299 |
| Down‐regulation | ||||
| Dr.3663.1.A1_at | dao.1 | −1.94 | −3.31 ± 0.11* | 3.59 E‐08 |
| Dr.25405.1.A1_at | sox19b | −1.80 | −2.09 ± 0.19* | 0.001 |
| Dr.14687.1.A1_at | copb2 | −1.39 | −1.69 ± 0.19* | 0.004 |
| Dr.3546.1.S1_at | wls | −1.25 | −1.67 ± 0.16* | 0.006 |
| Dr.3211.1.A1_at | grik‐l | −1.23 | −2.94 ± 0.21* | 2.53 E‐04 |
| Dr.8118.1.A1_at | otpb | −1.22 | −1.64 ± 0.23* | 0.004 |
Values of fold change by RT-qPCR are given as mean of fold change ± standard deviation. The study includes samples used for microarray hybridization and samples from independent treatments; a total of n = 8. For better comparison of the results obtained with both techniques, microarray and RT-qPCR, Morphine vs. Control fold changes are indicated with positive and negative values for up- and down-regulations, respectively. A one-way t-test was performed to determine whether fold changes obtained for morphine-regulated genes were different from 1 and significant values are indicated with asterisks.