Table 1.

Digest product ions of PilE from 278534 strain (from Figure 2).

Digestion Product	Measured m/z	Charge	Measured Monoisotopic Mass [M+H]+	Theoretical Monoisotopic Mass [M+H]+	Error (ppm)
[1–30]+Me	1088.2893	3	3262.8533	3262.8524	0.290
[31–44]	492.9174	3	1476.7376	1476.7363	0.912
[31–44]	738.8725	2	1476.7377	1476.7363	0.964
[31–44]	1476.7381	1	1476.7381	1476.7363	1.219
[31–44]Ox	746.8698	2	1492.7323	1492.7312	0.753
[31–44]Ox	1492.7366	1	1492.7366	1492.7312	3.618
[45–59]	844.4092	2	1687.8111	1687.8326	12.724*
[60–75]+DATDH+PG	928.9366	2	1856.8659	1856.8637	1.197
[60–75]+2DATDH+PG	1042.9924	2	2084.9775	2084.9747	1.354
[76–92]Ox	915.4706	2	1829.9339	1829.9313	1.434
[80–92]	676.8405	2	1352.6737	1352.6726	0.831
[80–92]+DATDH	790.8960	2	1580.7847	1580.7836	0.711
[93–112]+DATDH	739.7317	3	2217.1805	2216.1769	†
[93–112]+DATDH	1108.5933	2	2216.1793	2216.1769	1.094
[99–112]	701.3835	2	1401.7597	1401.7584	0.944
[99–112]	1401.7600	1	1401.7600	1401.7584	1.141
[99–112]+DATDH	815.4392	2	1629.8711	1629.8694	1.057
[113–121]+DATDH	651.2957	2	1301.5841	1301.5831	0.786
[113–121]+DATDH	1301.5850	1	1301.5850	1301.5831	1.460
[122–138]	604.2972	3	1810.8770	1809.8912	-*
[130–138]	555.2745	2	1109.5417	1109.5408	0.832

^*Errors for these ions are greater than expected since these digest products contain asparagine vicinal to glycine residues and are therefore subject to facile deamidation. For the ion at m/z 844.4092 deamidation is incomplete but the monoisotopic peak is very close to the baseline. For the ion at m/z 604.2972 complete deamidation increases the observed mass by approximately 1 Da.

^†The monoisotopic peak for this ion is obscured by the doubly charged [31–44] at m/z 738.8725.