Fig. 3.

P2X7 receptor is dispensable for P. gingivalis-induced TNF-α, IL-10, CXCL1 and IL-6 secretion. TNF-α (a), IL-10 (b), CXCL1 (c) and IL-6 (d) secretion were analyzed by ELISA in BMDM supernatants from WT and P2X7−/− mice in response to eATP stimulation. Culture supernatants were collected after 18 h of infection with P. gingivalis strains 381 or DPG3, or DPG3 and fimbriae (Fim; MOI of 100) followed by 5 mM of eATP for 30 min. Data are shown as means ± SD and are representative of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, vs. the indicated treatment group.