Table 5.
Mean (±SE) levels of altered acethylcholinesterase (AchE), non-specific esterases (α- and β-esterase), glutathione S-transferase (GST) and monooxygenase (MFO) activities of Ae. aegypti adults collected from different locations in Singapore
Strain | n | Mean enzyme activities 1 (Mean 2 ± SE) | ||||
---|---|---|---|---|---|---|
AchE | α-Esterase | β-Esterase | GST | MFO | ||
Bora-bora | 93 | 30.13 ± 0.67 | 21.51 ± 0.70 | 23.61 ± 0.74 | 0.04 ± 0.00 | 0.13 ± 0.01 |
Ang Mo Kio | 79 | 33.98 ± 0.44* | 22.23 ± 0.56 | 23.96 ± 0.81 | 0.06 ± 0.00 | 0.02 ± 0.00* |
Jurong East | 94 | 32.55 ± 1.08 | 2.08 ± 1.80* | 1.93 ± 1.69* | 0.05 ± 0.00 | 0.53 ± 0.02* |
Yishun | 82 | 46.35 ± 0.97* | 15.99 ± 0.42* | 19.67 ± 0.57* | 0.04 ± 0.00 | 0.22 ± 0.01* |
Chua Chu Kang | 81 | 38.31 ± 0.55* | 7.85 ± 0.32* | 10.60 ± 0.47* | 0.05 ± 0.00 | 0.14 ± 0.00* |
Clementi | 44 | 34.25 ± 1.49* | 17.78 ± 0.81* | 19.09 ± 1.03* | 1.99 ± 0.04* | 0.13 ± 0.01 |
Pasir Ris | 47 | 33.85 ± 0.66* | 9.54 ± 0.44* | 12.67 ± 0.55* | 0.04 ± 0.00 | 0.01 ± 0.00* |
Woodlands | 32 | 27.41 ± 1.10* | 13.75 ± 0.81* | 14.72 ± 0.96* | 0.04 ± 0.00 | 0.20 ± 0.01* |
1Enzyme activities are expressed as the reaction rate of different substrates/min/mg protein. 2Means followed by the asterisk are significantly different compared to the Bora-Bora strain (p < 0.05, two samples t-test or Mann–Whitney test). AchE: percentage insensitive acethylcholinesterase activity after propoxur inhibition. α-esterase: nmole of 1-naphthol/min/mg protein, β-esterase: nmole of 1-naphthol/min./mg protein. GST: mMole of CDNB/min/mg protein. MFO: nmole equivalent unit cyt P450/min/mg protein. The results in bold denotes activity that is more than two times above the control.