Skip to main content
. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: J Immunol. 2014 Sep 22;193(9):4303–4311. doi: 10.4049/jimmunol.1401505

Figure 4.

Figure 4

Bcl-3 contributes to BMDC maturation but is dispensable for inflammatory cytokine production. (A) WT and Bcl-3−/− BMDCs were left unstimulated or stimulated with LPS (100ng/ml). 24h later BMDCs were stained for markers indicated, and analyzed by flow cytometry, gated on CD11c+CD11b+ cells. Representative MFI plots in top rows, summaries for differentially expressed markers in bottom row. Isotype control staining is represented by shaded area. Data presented as mean ±SEM; n=10/group. (B) WT and Bcl-3−/− BMDCs (105) were stimulated with LPS for 18h in 96-well plates, and indicated cytokines present in supernatants were measured with CBA. Data shown as mean ±SEM; n=3-4/group. (C) WT, Bcl3−/− and Bcl3−/−/IL-10−/− BMDCs were loaded with OVA (100μg/ml) and stimulated with LPS (100ng/ml) o.n., and co-cultured with CFSE-labeled OT-II T cells for 72h. Cells were stained and gated for CD4, analyzed with flow cytometry and data shown as the mean ±SEM; n=2/group. (D) WT and Bcl-3−/− BMDCs were treated and seeded together with CFSE-labeled OT-II T cells as in (C) in bottom chambers of a transwell plate. LPS stimulated WT and Bcl-3−/− BMDCs were seeded in upper chambers, allowing for every combination of BMDCs in the two chambers as indicated. After 72h, cells in bottom chamber were analyzed as in (C). Data shown as mean ±SEM; n=4/group based on 2 experiments. *P<0.05, **P<0.01.