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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: J Immunol. 2014 Sep 22;193(9):4368–4380. doi: 10.4049/jimmunol.1400876

FIGURE 3. CTLA4 reduction increased effector memory formation of Treg cells in the target tissue of mice on diverse genetic backgrounds.

FIGURE 3

(A) Flow cytometry analyses of the Treg-EFF and Treg-EM subsets in the pancreas (numbers represent percentages of gated Treg populations). (B) Frequencies and total cell numbers of the CD4+ Treg-EM subset with CTLA4RNAi or transgene-negative littermate controls on the B6.H2g7 background (n=5–6 per group, 4–12 week old; Mean ± SEM). (C) Analyses of mice on the B6 background. Control data represent a pool of transgene-negative littermates or age- & sex-matched PL4 vector transgenic mice (n=4 per group, 9–16 week old; Mean ± SEM). (D) Flow cytometry plots of the PLN and pancreas in BDC2.5/NOD mice showing auto antigen-specific Treg-EFF and Treg-EM subsets (numbers represent percentages of gated Treg populations). (E) Comparison of the frequencies of antigen-specific CD4+ Treg-EM and CD4+ Treg-EFF subsets in the tissue and the draining lymph nodes of BDC2.5/NOD mice (n=7 per group; line indicating average of the group). (F) Frequencies and total cell numbers of the auto antigen-specific Treg-EM subset impacted by CTLA4 modulation. Control data represent a pool of CTLA4RNAi-transgene-negative littermate BDC2.5 mice or age- & sex-matched PL4/BDC2.5 mice (n=6–7 per group, 7–12 week old; Mean ± SEM). Each data-point represents one animal. *p<0.05; **p<0.01; ***p<0.005; ns, not significant.