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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: J Immunol. 2014 Sep 22;193(9):4368–4380. doi: 10.4049/jimmunol.1400876

FIGURE 7. CTLA4 down regulation increased IL17 and IFNγ production by auto antigen-specific CD4+ TEM cells.

FIGURE 7

(A) Representative intracellular flow cytometry plots of IL17- and IFNγ-producing autoimmune CD4+ TEM and CD4+ TEFF subsets in the PLN and pancreas in BDC2.5/NOD mice (numbers represent percentages of gated populations). (B) Frequencies of IL17- and IFNγ-producing autoimmune CD4+ TEFF and CD4+ TEM subsets in the PLN and pancreas in BDC2.5/NOD mice (n=6–11 mice per group). (C–H) The effects of CTLA4 modulation by RNAi (C, E and G) and by antibody treatment (D, F and H) on IL17-producing autoimmune CD4+ TEM cells (C–D), IFNγ-producing autoimmune CD4+ TEM cells (E–F) and IFNγ-producing autoimmune CD4+ TEFF cells (G–H) in the ILN, PLN and pancreas. Control data represent a pool of CTLA4RNAi-transgene-negative littermate BDC2.5 mice or age- & sex-matched PL4/BDC2.5 mice (n=4–10 mice per group). Each data-point represents one animal (Mean ± SEM). *p<0.05; **p<0.01; ***p<0.005.