Fig 3. Ssbp2 deletion affects the frequency of HSPC subpopulations.

A. Frequency of Lin⁻ cells is unaffected by Ssbp2^−/− BM. A representative image of gating within live population for lineage-negative cells B. Frequency of LSK cells was unaffected by Ssbp2^−/− BM. A representative image of c-Kit, Sca1 gating in Lin⁻ cells. C and E. Frequency of LMPPs (Flt3^hiCD34⁺ LSKs) is decreased in Ssbp2^−/− BM. C. Representative flow cytometry analysis showing LT-HSCs (Flt3⁻CD34⁻ LSKs), ST-HSCs (Flt3⁻CD34⁺ LSKs), MPPs (Flt3^loCD34⁺ LSKs), and LMPPs (Flt3^hiCD34⁺ LSKs) within LSKs in Ssbp2-null mice and their WT littermates. E. Frequency of LT-HSCs, ST-HSCs, MPPs and LMPPs within the LSK population in WT (n = 5) and null mice (n = 5). D, F and G. Frequency of CD48 cells is increased in Ssbp2^−/− BM. D. Representative flow cytometry analysis showing CD48⁺ cells within LSKs in Ssbp2-null mice and their WT littermates. F. Frequency of CD48⁺ and CD48⁻ population within the LSK compartment in WT (n = 5) and null mice (n = 5). G. Representative histogram for anti-CD48 staining. Note the increase in frequency in CD48⁻ cells in null mice. Data in E and F represent mean ± S.D from age-matched WT (n = 5) and null mice (n = 5)*p<0.05) as determined by unpaired, two tailed t test between WT and Ssbp2^−/− mice.