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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Acta Neuropathol. 2014 Jul 6;128(5):639–650. doi: 10.1007/s00401-014-1314-y

Fig. 2. Characterization of immunoaffinity-captured exosomes from human blood plasma.

Fig. 2

(a) Electron micrograph of anti-L1CAM-captured plasma exosomes (inset: immunogold labeling of L1CAM). (b) Western blot showing that Alix, a common exosome marker, and L1CAM were enriched with anti-L1cam capture, but not with normal IgG capture. (c) α-Synuclein (syn) levels in anti-L1CAM-captured plasma exosomes were measured using a Luminex immunoassay, compared to the levels in normal mouse IgG-captured (mIgG) or “Empty” (no bead “capture”) samples. (d) Specificity was also confirmed by using exosome-poor plasma (supernatant after ultracentrifugation). Aliquots from the same pooled samples were used in these experiments (b-d) for comparison.