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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Mol Cancer Res. 2014 Jun 25;12(10):1460–1469. doi: 10.1158/1541-7786.MCR-14-0038

Figure 3. Inhibition of basal ERK activity in H226, MSTO211H and H2452 cells by ponatinib.

Figure 3

The indicated cell lines were incubated in serum-free medium for 2 hours in the presence of increasing concentrations of ponatinib. Cell extracts were prepared and immunoblotted for phospho-ERK and as described in the Materials and Methods. The filters were stripped and reprobed for total ERK to verify equal protein loading.