TABLE 1.
Ponatinib sensitivity associates with FGF2 and FGFR1 expression in mesothelioma cell lines
| Cell Line | Cell Line Morphology/ Histology |
Ponatinib IC50, nM |
FGFR1 protein |
FGFR1:C EP8 ratio (FISH) |
FGFR1 GCN |
FGF2 mRNA (QPCR) |
FGF2 protein (pg/µg cell protein) |
|---|---|---|---|---|---|---|---|
| H226 | Epithelial mesothelioma | 6 | 5.4 | 1.0 | 1.1 | 0.24±.07 | 438±15 |
| MSTO211H | Biphasic mesothelioma | 7 | 4.2 | 1.7 | 0.88 | 0.76±.22 | 888±139 |
| H2452 | Biphasic mesothelioma | 50 | 2.0 | 1.2 | 1.91 | 0.12±.02 | 249±54 |
| Met5A | Immortalized non-malignant mesothelial cells | 100 | 1.4 | ND | ND | 0.27±.05 | 191±6 |
| H290 | 200 | 1.3 | 0.9 | ND | 0.17±.06 | 96±5 | |
| H28 | mesothelioma | 500 | 1.1 | ND | 0.8 | 0.26±.07 | 133±15 |
| H513 | Epithelial mesothelioma | 500 | 0.1 | 1.2 | ND | 0.00±.00 | 0±4 |
| H2052 | mesothelioma | 500 | 0.4 | 0.8 | 0.7 | 0.97±.26 | 690±54 |
| H1703 | NSCLC | - | - | 3.5 | 3.0 | - | - |
The ponatinib IC50 values were calculated from the data in Figure 2 and FGFR1 protein levels were determined by immunoblotting and densitometry. The indicated cell lines were submitted to 2 color FISH assays for FGFR1 and CEP8 and the ratios are tabulated. The Cancer Cell Line Encyclopedia SNP data was interrogated and the relative FGFR1 gene copy number (GCN) is presented. FGF2 mRNA and protein were measured by quantitative RT-PCR (Materials and Methods) and ELISA (R&D Systems, Minneapolis, MN), respectively, with mean and SEM presented.