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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: J Immunol. 2014 Sep 26;193(9):4580–4589. doi: 10.4049/jimmunol.1400699

Figure 3. CD1d-dependent stimulation of a sulfatide-reactive type II NKT cell by self-lysophospholipids.

Figure 3

(A) A type II NKT Hy19.3 or a type I NKT Hy1.2 were stimulated in the presence of APCs (irradiated splenocytes) from either the wild-type C57BL/6 (CD1d+/+) or CD1d-deficient (CD1d−/−) mice in the presence of different concentration of lipids (0.6 – 20 μg/ml). IL-2 release at optimum concentration (5 μg/ml) of indicated lipids is shown. (B) A typical dose titration of Hy19.3 stimulation in the presence of APC and LPC (0.6 – 20 μg/ml) is shown. These data are representative of at least 3 independent experiments.