FIGURE 1.
Complement opsonization of HIV-1 results in reduced inflammatory responses in DCs compared with F-HIV. DCs (1 × 106/ml) were exposed to an MOI of 8 of F-HIV, C-HIV, or CI-HIV or were mock treated and evaluated for expression of IL-1β (A) and IL-6 (B) by lysing the cells and performing qPCR. Concentrations of IL-1β, IL-6, CXCL8, IL-12, and TNF-α were also measured in supernatants collected from DC cultures 24 h after exposure using a flow Cytometric Bead Array (C). qPCR values have been normalized with the mock value set to 1. Data are shown as means ± SEM of 4–12 independent experiments. *p < 0.05 compared with F-HIV.