FIGURE 5.

Complement opsonization of HIV-1 suppressed TLR8 activation in DCs. DCs (1 × 10⁶/ml) were pretreated with the weak base NH₄Cl, the TLR8 specific inhibitory oligonucleotide IRS957, or control before exposure to an MOI of 8 for F-HIV or C-HIV or were mock treated for 6 h. mRNA levels of IL-1β and MxA for DCs pretreated with NH₄Cl (A and B) or IRS957 (C and D) were assessed by qPCR. Values have been normalized with mock values set to 1, and data are shown as means ± SEM of three to eight independent experiments. *p < 0.05.