Table II. Protein expression of inflammatory and antiviral factors in DCs 24 h after exposure to F-HIV, C-HIV, or CI-HIV.
| F-HIV |
C-HIV |
CI-HIV |
||
|---|---|---|---|---|
| Inflammatory factors | ||||
| CCL5 | C-C motif chemokine 5 | 2.40* | 1.40 | 1.50 |
| CCL17 | C-C motif chemokine 17 | 2.60* | 1.63 | 2.10 |
| CCL22 | C-C motif chemokine 22 | 1.73 | 1.33 | 1.83 |
| EBI3 | IL-27 subunit β | 4.33* | 3.93 | 4.17 |
| CD40 | TNFR superfamily member 5 | 2.27 | 1.63 | 1.63 |
| TRAF1 | TNFR-associated factor 1 | 5.40* | 3.87 | 4.07 |
| TNFAIP2 | TNF-α–induced protein 2 | 3.83* | 2.50 | 2.13 |
| NFKB1 | NF-κB p105 subunit | 2.27* | 2.07* | 2.10* |
| NFKB2 | NF-κB p100 subunit | 2.33* | 2.07* | 2.09* |
| Antiviral factors | ||||
| MX1 | IFN-induced GTP-binding protein Mx1 | 7.83* | 4.50 | 3.40 |
| MX2 | IFN-induced GTP-binding protein Mx2 | 4.83* | 2.90 | 2.20 |
| GBP1 | IFN-induced guanylate-binding protein 1 | 2.83* | 1.90 | 1.43 |
| GBP2 | IFN-induced guanylate-binding protein 2 | 1.8* | 1.43 | 1.23 |
| IFIT1 | IFN-induced protein with tetratricopeptide repeats 1 | 5.77* | 2.97 | 2.50 |
| IFIT2 | IFN-induced protein with tetratricopeptide repeats 2 | 6.07 | 3.90 | 2.97 |
| IFIT3 | IFN-induced protein with tetratricopeptide repeats 3 | 7.33* | 4.47 | 3.40 |
| IFIT4 | IFN-induced protein with tetratricopeptide repeats 4 | 2.65 | 1.80 | 1.55 |
| PKR | IFN-induced, dsRNA-activated protein kinase | 3.17 | 1.70 | 1.57 |
| IFIH1 | Isoform 1 of IFN-induced helicase C domain–containing protein 1 | 3.97* | 2.43 | 1.90 |
| ISG15 | Ubiquitin-like protein ISG15 | 15.73* | 8.9 | 6.00 |
DCs (1 × 106/ml) were exposed to an MOI of 8 of F-HIV, C-HIV, or CI-HIV or were mock treated for 24 h and expression of proteins associated with inflammatory and antiviral responses was determined by mass spectroscopy proteomics. Values have been normalized with mock set at 1 and are shown as the mean fold increase compared with mock of three independent experiments.
*p < 0.05 upregulation compared with mock.