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. 2014 Oct 2;10(10):1116–1127. doi: 10.7150/ijbs.10236

Figure 1.

Figure 1

Conditional knockout of SRC-3 in prostatic epithelial cells of TRAMP mice. A. Deletion of the floxed SRC-3 alleles in the prostates of 3PEKOT mice. Genomic DNA samples were prepared from prostate tumors of 24-week-old 3FloxT (n=3) and 3PEKOT (n=3) mice or from a WT mouse prostate and used as templates for PCR. DNA fragments representing the PB-SV40-T/t and PB-Cre transgenes and the WT, floxed and deleted SRC-3 alleles were amplified using allele-specific primer pairs. B. IHC for SRC-3 (brown) in the prostate sections prepared from 8-week-old 3FloxT and 3PEKOT mice. C. IHC for SV40 T antigen (brown) in the prostate sections prepared from 8-week-old 3FloxT and 3PEKOT mice. The percentage of SV40 T-positive was calculated by dividing the number of positive epithelial cells by the number of total epithelial cells per imaging field. Three random imaging fields per section and sections from 5 mice were assessed.