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The basal pERK1/2 levels in HEK293T cells transiently transfected with WT or mutant MC4Rs with increased basal pERK1/2 levels (A), or decreased basal pERK1/2 levels (B and C). Results are expressed as percentage of the WT basal pERK1/2 level and represent the mean ± SEM of five independent experiments. * indicates significant differences (P < 0.05).
