Figure 8.

Crosstalk among C3a, C5a, and TGF-β1. SAECs were treated with or without C3a or C5a (100 nM) for the indicated times. A, B) TGFb (A) and SMAD7 (B) were analyzed by quantitative reverse transcriptase PCR (qRT-PCR; endogenous control, GAPDH). Values represent means ± sem (n=3). *P < 0.05; unpaired t test. C) SAECs were treated with or without TGF-β1 (10 ng/ml) for the indicated times. Cell lysates were immunoblotted against specific receptors C3aR and C5aR (β-actin, loading control). D, E) SAECs were pretreated for 1 h with C3aRA (SB209157; 50, 100 μM; D) or C5aRA (PMX205; 1, 5 μM; E) and then treated with or without C3a or C5a (100 nM) for 6 h, respectively. Cell lysates were immunoblotted for CD46, CD55, and Snail (β-actin, loading control). Data are representative analyses of cells from 3 independent donors.