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. 2014 Oct;28(10):4369–4380. doi: 10.1096/fj.14-252890

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Figure 2. — In vivo Cyr1 Inhibition causes a respiratory phenotype. A) Wild-type W303 yeast were incubated for 20 h in medium containing either glucose (YPD) or ethanol/glycerol (YPEG) in the presence of the indicated concentrations of MDL-12330A or KH7. Culture density was measured as OD₆₀₀, and data are reported as a percentage of DMSO control. B) Growth curve of wild-type W303 yeast in YPEG medium in the presence of 50 μM of MDL-12330A or KH7. C) KCN-sensitive endogenous cell respiration of wild-type W303 yeast measured polarographically in the presence of 50 μM of MDL-12330A or KH7. D) NADH-driven oxidation in coupled (+ADP) and uncoupled (+CCCP) mitochondria in the presence of the indicated concentrations of MDL-12330A or KH7. Bars represent means ± sd. *P < 0.05.