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. 2014 Oct;28(10):4324–4334. doi: 10.1096/fj.14-252684

Figure 1.

Figure 1.

Ouabain inhibits sFlt1 production in primary cytotrophoblast cells and villous explants from normal pregnancy. A, B) Ouabain inhibits sFlt1 production in a dose-dependent (A; n=6) and time-dependent manner (B; n=4) in culture supernatants of cytotrophoblasts from normal pregnancy. Data are expressed as a percentage of the sFlt1 (pg/ml) levels in controls; average amount of sFlt1 produced by normal cytotrophoblast (control) was 1974 ± 1076 pg/ml (A). *P < 0.05 vs. control; 1-way ANOVA with Dunn's multiple-comparison test. **P < 0.05 vs. control; 2-way ANOVA, repeated measures for the analysis of data. C) Similar dose-dependent reduction of sFlt1 is observed in culture supernatant of villous explants (n=3) from normal pregnancy. Average amount of sFlt1 produced by normal placental villous explant (control) was 771 ± 95 pg/ml. D) Effects of ouabain (500 nM) on sFlt1 protein levels by Western blots in culture supernatant of villous explants (n=3) enriched by heparin-agarose chromatography (top panel using Sigma-Aldrich antibody; bottom panel using R&D antibody). E) Quantification of the Western blots for Sigma-Aldrich antibody. *P < 0.05 vs. control; Student's t test.