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. 2014 Oct;28(10):4324–4334. doi: 10.1096/fj.14-252684

Figure 2.

Figure 2.

Ouabain inhibits sFlt1 production in preeclamptic cytotrophoblasts and villous explants. A, B) Ouabain inhibits sFlt1 production in a dose-dependent manner in cytotrophoblasts (A; n=6) and in villous explants (B; n=3) from preeclamptic pregnancies. Data are expressed as a percentage of the sFlt1 (pg/ml) levels in controls. Average amount of sFlt1 produced by preeclamptic cytotrophoblasts (control) was 1556 ± 717 pg/ml (A); average amount of sFlt1 produced by preeclamptic placental villous explant (control) was 2106 ± 547 pg/ml (B). *P < 0.05 vs. control, 1-way ANOVA with Dunn's multiple-comparison test. C) Effects of ouabain (500 nM) on sFlt1 protein levels in culture supernatant of preeclamptic explants (n=3) enriched by heparin-agarose chromatography top panel using Sigma-Aldrich antibody; bottom panel using R&D antibody). D) Quantification of the Western blots for Sigma-Aldrich antibody. *P < 0.05 vs. control; Student's t test.