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. 2014 Oct;28(10):4324–4334. doi: 10.1096/fj.14-252684

Figure 3.

Figure 3.

Ouabain treatment leads to concurrent decrease in HIF-1α and sFlt1 levels. A) Reduced HIF-1α and sFlt1 protein levels in explants from normal and preeclamptic placentas (n=6/group) treated with 500 nM ouabain. B, C) Quantification of Western blots for HIF-1α (B) and sFlt1 (C). D) Nuclear HIF-1α of explants (n=3) treated with 500 nM of ouabain is significantly down-regulated compared to controls. E) Quantification of Western blot. F) HIF-2α levels in NP (n=3) and PE (n=3) explants treated with 500 nM of ouabain show a significant decrease in HIF2-α compared to controls. NP, normal pregnancy; PE, preeclampsia. G) Quantification of Western blots. *P < 0.05 vs. control; Student's t test.