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. 2014 Oct;28(10):4408–4419. doi: 10.1096/fj.14-253971

Figure 2.

Figure 2.

TFAM KO efficiency in iWAT, eWAT, and BAT and effect on oxphos expression and function. A) TFAM and mRNA of mitochondria-encoded genes, according to qPCR, in isolated adipocytes from iWAT, eWAT, and BAT from 10- to 12-wk-old male control (Lox) and adipo-TFAM-KO (KO) mice. Data are means ± sem of 6 samples/group. *P < 0.05. B) Citrate synthase (Cs) activity was assessed from isolated mitochondria of iWAT, eWAT, and BAT adipocytes. Cells were isolated from 10- to 12-wk-old male Lox and KO mice. Data are means ± sem of 7 samples/group. *P < 0.05. C) Expression of TFAM and oxphos members was analyzed by Western blot from mitochondria isolated from iWAT, eWAT, and BAT adipocytes of 12- to 15-wk-old male KO and Lox mice. Data are representative of 4 samples/genotype. D) Isolated mitochondria respiratory chain enzyme activities were assessed in mitochondria isolated from white and brown adipocytes (n=7/genotype). Cx, complex. *P < 0.05.