Table 3.
Open probability and kinetics of MscL channels from different organisms
| Organism | Negative pressure |
Negative pressure and LPC |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| P1/2 (mmHg) | τ1 (ms) | τ2 (ms) | Conductance, G (nS) | P1/2 (mmHg) | τ1 (ms) | τ2 (ms) | τ3 (ms) | Conductance, G (nS) | |
| M. tuberculosis | 155.75 ± 0.29 | <1 | <1 | 3.26 ± 0.10 | 35.06 ± 0.07 | <1 | 6.03 ± 1.47 (27) | 17.60 ± 7.36 (28) | 3.18 ± 1.09 |
| L. lactis | 142.50 ± 1.76 | <1 | 1.85 ± 0.18 (38) | 1.80 ± 0.27 | 78.24 ± 0.50 | <1 | 2.68 ± 0.21 (37) | 31.16 ± 3.50 (3) | 1.85 ± 0.33 |
| E. coli | 95.17 ± 0.33 | <1 | 3.95 ± 0.08 (49) | 3.70 ± 0.23 | 34.63 ± 0.48 | <1 | 8.73 ± 0.15 (41) | 47.82 ± 0.26 (23) | 3.92 ± 0.33 |
Data are means ± se (% normalized population). Open probability, dwell time, and single-channel conductance of MscL homologs activated by negative pressure or LPC plus with negative pressure are shown. The P1/2 of Ec-MscL is the lowest in the presence of negative pressure only, followed by Ll-MscL and Tb-MscL. On the other hand, activation of channels by negative pressure and 9.5 μM LPC has an overall significant impact on the P1/2 and dwell times of all MscL homologs, but the change is the greatest for Tb-MscL.